Total Nitrogen (Plant)

Dumas method

This technique uses a LECO FP-528 Nitrogen Analyzer to determine total N in plant materials. A 150 mg sample is weighed into a gel capsule and dropped into an 850o C furnace purged with O2 gas. The combustion products of CO2, H2O and NOx are filtered, cooled by a thermoelectric cooler to condense most of the water, and collected in a large ballast. A 3 cc aliquot of the ballast combustion products is integrated into a He carrier stream and passed through: 1.) a hot copper column where the O2 is removed and the NOx gasses are converted to N2 2.) a reagent tube which scrubs the CO2and remaining H2O from the stream. The N2 content is then measured by a thermal conductivity cell against a He background and the result displayed as weight percentage of nitrogen.

(See Method References No. 60 and No. 61)

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Plant Analysis